Italy boss Marcello Lippi has confirmed his 23-man squad by sending reserve defender Daniele Bonera home on Sunday.
The move comes 24 hours before the Azzurri begin their Group E campaign against Ghana in Hannover on Monday evening.
Parma man Bonera remained with the squad in Germany amid growing concerns that Gianluca Zambrotta would not overcome a leg injury.
However, the Juventus defender is on course to be available for Italy's second group game against USA in Kaiserslautern next Saturday, meaning there is no place for Bonera in Lippi's squad.
Meanwhile, Fiorentina striker Luca Toni says it is a dream for him to appear in a World Cup for Italy.
The former Palermo hit-man, who became the first player to score over 30 goals in Serie A last season, finds his meteoric rise somewhat hard to believe.
"You would have to have been completely crazy to think that when I was in Serie B, I could end up at the World Cup," Toni said.
"But that is the beauty of football, these things can really happen.
"There is a huge desire on our part to just get out there and put all our work in to practice.
"I've never been at an event like this and so it is really exciting for me."