Paraguay manager Gerardo Martino has named his preliminary squad for this summer's World Cup finals, with Roque Santa Cruz and Paulo da Silva earning call-ups.
Borussia Dortmund's Argentine-born striker Lucas Barrios has also been included in the 30-man line-up after he was recently nationalised.
Barrios' squad that will be cut to 23 players at the end of May in time for the start of the finals on 11th June, when Paraguay will compete in Group F alongside Italy Slovakia and New Zealand.
"Nobody can doubt the capabilities of Lucas Barrios," said Martino, who played for Argentina at international level.
Barrios, whose mother is Paraguayan, has enjoyed a fine season with Dortmund, while fellow strikers Oscar Cardozo of Benfica and Manchester City's Santa Cruz have also featured.
"I can imagine Paraguay causing some surprises in South Africa," said Barrios, who has scored 18 goals in the Bundesliga this season.
Sunderland defender Da Silva, Atalanta midfielder Edgar Barreto and Wolfsburg's Jonathan Santana were featured among the players who ply their trade in Europe at club level
Goalkeepers: Justo Villar (Valladolid), Aldo Bobadilla (Deportivo Independiente), Diego Barreto (Cerro Porteno)
Defenders: Claudio Morel Rodriguez (Boca Juniors), Denis Caniza (Leon), Paulo da Silva (Sunderland), Dario Veron (Pumas), Julio Cesar Caceres (Atletico Mineiro), Julio Manzur (Tigres), Carlos Bonet (Olimpia), Aureliano Torres (San Lorenzo de Almagro), Marcos Caceres (Racing), Antolin Alcaraz (FC Bruges)
Midfielders: Edgar Barreto (Atalanta), Cristian Riveros (Cruz Azul), Osvaldo Martinez (Monterrey), Victor Caceres (Libertad), Enrique Vera (Liga Deportiva Universitaria), Jonathan Santana (Wolfsburg), Sergio Aquino (Libertad), Eduardo Ledesma (Lanus), Nestor Ortigoza (Argentinos Juniors), Marcelo Estigarribia (Newell's Old Boys)
Forwards: Roque Santa Cruz (Manchester City), Nelson Haedo Valdez (Borussia Dortmund), Oscar Cardozo (Benfica), Edgar Benitez (Toluca), Lucas Barrios (Borussia Dortmund), Rodolfo Gamarra (Libertad), Jorge Achucarro (Newell's Old Boys)