The top three women's seeds are all out of the French Open after Agnieszka Radwanska lost 6-4 6-4 to Ajla Tomljanovic in the third round on Friday.
While not as big a shock as either Serena Williams or Li Na's exits, it was a sizeable upset nonetheless and leaves fourth seed Simona Halep as the highest-ranked player remaining in the tournament.
Radwanska has never enjoyed playing on clay, particularly in the slow and heavy conditions that have been a feature of the first week at Roland Garros this year.
She reached the quarter-finals for the first time last year but was on the back foot from the start against 21-year-old Croat Tomljanovic.
It was a breakthrough at the top level for the Croatian world No 72, who is through to the fourth round at a grand slam for the first time at her maiden French Open.
This has been a tournament where the young guns have made their mark, and the powerful Tomljanovic joins Garbine Muguruza, Kristina Mladenovic and Taylor Townsend as a headline maker.
Tomljanovic, Muguruza and Mladenovic, the conquerors of the world's top three in Paris, were all born in 1993.
The Croatian said: "After seeing the two first seeds go out, you kind of feel like 'I can do this too'. I grew up with these girls that are beating them.
"I went on the stadium for the first time, and she kind of feels like home there, because she's been there a lot more than I did. I went out there, and I really, inside really thought I could win. I think that showed and it is why I won."
Tomljanovic moved to America eight years ago to train at Chris Evert's academy and remains close with the 18-time grand slam singles winner.
"She really wants me to do well, and I can feel that from her," Tomljanovic said. "I'm really happy that she's someone I can rely on."
Radwanska pulled the first set back from 5-1 to 5-4 but Tomljanovic held on and then moved ahead early in the second.
"I don't think was my day today," said Radwanska. "I think I just started too slow. I was 5-1 down, and I guess I was a little late for the first set. I was still trying, I almost came back, but it didn't work out well.
"But she definitely played good tennis today. I had my chances. I didn't take them. I think that cost me the match."
If Maria Sharapova is feeling any pressure from being the favourite then Paula Ormaechea can attest the Russian is not showing it following a 6-0 6-0 thrashing.
The 2012 champion and 2013 finalist has transformed herself into the second best player in the world on clay and needed just 51 minutes to thump Ormaechea in the third round.
It all looked so different when Ormaechea forced Sharapova to save three break points in a nine-minute opening game that was played either side of a short rain break.
But after missing those chances, it all went away from the Argentinian very quickly as Sharapova found her groove.
The Russian is an utterly ruthless competitor and barely allowed her opponent a sniff of another game to set up a fourth-round clash with 2010 finalist Sam Stosur.
The Australian knocked out ninth seed Dominika Cibulkova and could be a threat to Sharapova, but the seventh seed has won 13 of her previous 15 meetings with Stosur, including all three on clay.
Elsewhere, Spain's Carla Suarez Navarro (14) brushed aside Taylor Townsend of the United States with ease 6-2 6-2.