Third seed Karin Knapp crashed out of the Caro Open Colsanitas in the opening round in Bogota.
The Italian was an early victim as she lost 6-3 7-6 (7-4) on the opening morning on the tournament.
The other seeds in action came through unscathed as sixth-ranked Vania King beat Tadeja Majeric 6-1 7-5 and eighth seed Lourdes Dominguez Lino was a 6-2 6-4 winner over Teliana Pereira.
There were also first-round wins for Alexandra Panova and Irina Khromacheva.
Panova held her never after a second set wobble to beat Kristina Mladenovic 6-1 4-6 6-2, while lucky loser Khromacheva powered to a 6-2 6-2 win against Olivia Rogowska.