An injury to Flavia Pennetta ended Italy's hopes of reaching the final of the Hopman Cup in Perth.
Pennetta was forced to withdraw from her singles match against teenager Eugenie Bouchard as Canada kept alive their chances of progressing to the next stage with a 3-0 victory in Group A.
With the start of the Australian Open just 11 days away, Pennetta decided to pull out after losing the opening four games against Bouchard.
The world No 31 had an operation on the right wrist during 2012 but is confident this latest setback is nothing more than inflammation.
She had been close to withdrawing before the start of the match, with the injury clearly affecting her serve and forehand on Thursday.
The 31-year-old Italian is still hoping to play in Hobart next week before heading to the opening Grand Slam of the year in Melbourne.
Pennetta said: "It's the wrist that I had the operation on. Sometimes it gives me some problems but I hope it's nothing too serious.
"I was thinking maybe with some warm-up it would get better but it did not.
"I will have some treatment. Maybe I won't play for one or two days to help because I think it's more something like inflammation, it's not like a tear or ligaments."
Canada wrapped up a 3-0 victory after Milos Raonic beat Andreas Seppi in the men's singles, while the mixed doubles was forfeited because of Pennetta.