Sergiy Stakhovsky fell from cloud nine on Friday as the man who dethroned Roger Federer was beaten in four sets by Austrian Jurgen Melzer on a drizzly Court Three.
Ukraine's world No. 116 disappeared from the limelight almost as quickly as Rafa Nadal's conqueror Belgian Steve Darcis, as he slumped to a 6-2 2-6 7-5 6-3 defeat by the world No 37.
Bulgarian 29th seed Grigor Dimotrov is also out after losing his second round match 3-6 7-6 (7-4) 3-6 6-4 11-9 to Slovenian Grega Zemlja.
But world No 4 David Ferrer avoided any mishaps as he moved into the third round with a straight sets victory over fellow Spaniard Robert Bautista Agut. He will face Ukrainian Alexandr Dolgopolov in the third round.
When fourth seed Ferrer claimed the first set after 43 minutes, there was no hint that the 60th-ranked Bautista Agut could upset the country's pecking order and reach the third round of a grand slam for the first time.
But his clever shot-making drew a string of unforced errors from this year's French Open finalist in the second set and he tenaciously dug-in to take the third set to a tiebreak.
However, Ferrer kept his nerve to regain the lead, broke in the 11th game of the fourth set and closed it out to set up a third-round match against Ukraine's Alexandr Dolgopolov.
Pole Jerzy Janowicz, the 24th seed lurking dangerously in the bottom half of the draw, gave a vivid demonstration of his talent on Centre Court to reach round four for the first time, overpowering Spanish 15th seed Nicolas Almagro to set up a clash with Melzer.
There were also positive results for Tommy Haas, who beat Jimmy Wang 6-3 6-2 7-5 and Dolgopolov, who beat Santiago Giraldo 6-4 7-5 6-3.