Sixth seed Jo-Wilfried Tsonga retired from his Wimbledon second-round match against Latvia's Ernests Gulbis with a knee injury.
The Frenchman, who was a potential quarter-final opponent for Britain's Andy Murray, called it quits when trailing by two sets to one on Centre Court.
Tsonga received taping on his left knee at the end of the second set but, after dropping the third set to fall 6-3 3-6 3-6 behind, he was unable to continue.
"I tried, but no chance for me to beat a guy like this without my legs," he said.
The two-time semi-finalist joined a growing list of injury casualties on day three of Wimbledon; 10th seed Marin Cilic, 18th seed John Isner, Rafael Nadal's conqueror Steve Darcis and Czech veteran Radek Stepanek had earlier all been forced out.
In the women's draw, world number two Victoria Azarenka was unable to start her second-round match due to a knee problem and, moments after Tsonga's retirement, Kazakhstan's Yaroslava Shvedova withdrew with a right arm injury, handing 2011 champion Petra Kvitova a walkover.