Laura Robson's Eastbourne campaign came to an end after she suffered a 6-4 6-4 defeat to Caroline Wozniacki in the second round of the WTA Aegon International.
And she was followed out of the tournament by her fellow Briton Heather Watson, who was beaten in three sets by Russia's Elena Vesnina.
British No 1 Robson was in action against former World No 1 Wozniacki just three weeks after suffering defeat to her at the French Open.
But the 19-year-old failed to take advantage of the Dane's poor form in Paris and did not make amends on the south coast.
Robson, preparing under temporary coach Miles Maclagan for what will be her 11th Grand Slam appearance, continued where she had left off after dispatching Ukrainian qualifier Yuliya Beygelzimer on Tuesday.
She outplayed Wozniacki in the opening set but was made to pay making too many unforced errors.
The decisive moment came in the ninth game, with a pair of double faults in quick succession allowing Wozniacki to break for 5-4 and then serve for the set.
It was a pattern that continued in the first game of the second set, with three consecutive mistakes helping Wozniacki to come from 40-15 behind to break Robson.
The 2008 Wimbledon girls' singles champion responded, showing good composure to break back in the fourth game to make it 2-2.
However, Robson was eventually undone in the ninth game as Wozniacki broke again on her way to victory.
"You have to look at the positives ahead of next week," said Robson. "I'm going to get back to Wimbledon and have a good couple of days' practice before the first round."
Meanwhile, British No 2 Watson went down to a frustrating 6-1 3-6 6-2 loss against Vesnina.
Watson was broken by her Russian opponent in just the third game and the 21-year-old failed to recover in the first set, going down 6-1.
Watson showed impressive character and strength to take the second set, only to be undone in the deciding set.
Vesnina raced into a 5-0 lead after converting two break points before closing out the decider 6-2.
"I was in and out of focus," said Watson. "I thought the first three games I was playing well and then I just switched off for the rest of that set. It went so quick."