Elena Baltacha crashed out of the WTA Aegon International after losing a three set battle against world No 10 Maria Kirilenko.
The Wimbledon wild card went down 4-6 6-4 6-3 in a match which lasted two hours and 33 minutes on the South Coast.
On Sunday the 29-year-old Briton won her first title since recovering from a career-threatening injury, taking the Aegon Challenge crown in Nottingham.
But she could not follow that up against the French Open quarter-finalist from Russia with her hopes coming to an end at the round of 16 juncture.
Baltacha showed great fighting spirit against the Russian sixth seed and raced into a 4-1 lead after breaking twice at Devonshire Park.
Kirilenko responded by breaking for 4-2, although she could not stop Baltacha deservedly taking the first set on Court Two.
The second set was a much more even affair and was only taken by the Russian after converting a break point at the eighth attempt.
Baltacha received treatment for a back problem before the final set got off to a blistering start, with both players breaking at the first attempt.
The Scot squandered two break points at 3-3 and Kirilenko took full advantage, taking control and clinching victory.
"It was the perfect preparation for next week, definitely," Baltacha said.
"I am excited about it.
"The draw obviously comes out on Friday and there are a couple of players I'd
like to avoid, to be honest, but whatever my fate is, it is. That's it.
"If someone had said to me eight weeks ago that you're going to win Nottingham and then you're going to get a shot at Kirilenko at the second round of Eastbourne, I don't think I would have believed them."
Baltacha was followed out of the tournament by British No 1 Laura Robson, who was beaten by Caroline Wozniacki while Heather Watson exited in three sets to Elena Vesnina.
In the shock result of the day, Belgium's Yanina Wickmayer upset fourth-seeded Czech Petra Kvitova in three sets with the 2011 Wimbledon champion going down 3-6 6-4 7-5.