Joe Tomane and Chris Feauai-Sautia will miss Australia's third Bledisloe Cup Test against New Zealand on Saturday.
Tomane, a try-scorer in the 54-17 rout of Argentina last time out, and Feauai-Sautia had been set to start the match in Dunedin.
However they have been ruled out through hamstring injuries - joining Christian Leali'ifano and Scott Fardy in missing the match.
Waratahs winger Peter Betham has been called up as cover however Tomane and Feauai-Sautia are expected to be fit for the five-match tour of Europe.
Wallabies head coach Ewen McKenzie will announce his line-up for Saturday's clash, which will be shown live on Sky Sports 4 HD, on Thursday.