James Hook, Ryan Jones, Matthew Rees and Lee Byrne have been left out of the Wales squad for their two-Test tour of Japan this summer.
With 15 senior players named in the British & Irish Lions touring party, interim coach Robin McBryde has named 11 uncapped players in an initial 32-man training uad for the tour.
McBryde has confirmed that he checked with Warren Gatland that omitting the senior Wales men would not affect their chances of being given a call up as a replacement on the Lions tour.
"With those experienced players (who have been left out), we have had assurances that in omitting them from this squad their selection as standby players for the Lions is in no way affected. They will still be available should the call come.
"With that in mind we decided to develop these youngsters and take them on to the next level."
Five will be cut from the list with only 27 going on tour. Dan Biggar, Rhys Priestland, Bradley Davies, Scott Williams, Craig Mitchell, Ryan Bevington and Ken Owens are included to provide an experienced backbone to the squad.
McBryde said: "We have selected a squad with the right blend of experience and youth and this is a great opportunity for players to make their mark on the international stage.
"It's a chance for some players to experience the international environment for the first time but it's also an opportunity for players who have been involved previously to take the next step as we look to develop leaders.
"We are travelling to Japan as Six Nations Champions and are focused on the two-Tests that await us there and we will be looking to finish the season on a high."
Backs: S Shingler (London Irish), T Prydie (Newport Gwent Dragons), H Robinson, (Cardiff Blues), Liam Williams (Scarlets), D Howells (Ospreys), J Spratt, (Ospreys), O Williams (Cardiff Blues), A Beck (Ospreys), S Williams (Scarlets), D Biggar (Ospreys), R Priestland (Scarlets), Lloyd Williams (Cardiff Blues), T Knoyle (Scarlets), A Davis (Scarlets).
Forwards: R Bevington (Ospreys), R Gill (Saracens), S Hobbs (Cardiff Blues), S Andrews (Cardiff Blues), C Mitchell (Exeter), R Jones (Scarlets), K Owens (Scarlets), E Phillips (Scarlets), S Baldwin (Ospreys), B Davies (Cardiff Blues), L Reed (Cardiff Blues), A Coombs (Newport Gwent Dragons), J King (Ospreys), A Shingler (Scarlets), D Baker (Ospreys), R McCusker (Scarlets), A Pretorius (Cardiff Blues), J Navidi (Cardiff Blues).