Uncapped trio Joe Launchbury, Mako Vunipola and Tom Youngs have been included in England's squad to play Fiji on Saturday.
Wasps forward Launchbury and Saracens prop Vunipola made the final cut after England head coach Stuart Lancaster announced his retained list for the Twickenham game.
Launchbury only linked up with the squad on Monday as cover for the injured Tom Palmer and he has been preferred to Saracens lock Mouritz Botha and Harlequins' George Robson.
Leicester forward Tom Youngs also got the nod but Launchbury's club team-mate James Haskell was omitted from the final matchday squad.
Lancaster is expected to confirm his starting XV and eight-man bench for the game on Thursday morning.
"We have had a good two days back at Pennyhill Park and the players have been very focused on delivering against Fiji," said Lancaster.
"Saturday is a great opportunity for us to put a marker down and deliver a quality performance.
"It's great to have young players such as Mako Vunipola and Joe Launchbury get their opportunities in the match-day 23 and we are excited about the potential of the squad.
"Under the agreement with Premiership Rugby we are releasing some players back, including Alex Corbisiero, who will play for London Irish and we are looking forward to seeing how he goes."
Forwards: D Cole (Leicester Tigers), T Johnson (Exeter Chiefs), J Launchbury (London Wasps), J Marler (Harlequins), T Palmer (London Wasps), D Paice (London Irish), G Parling (Leicester Tigers), C Robshaw (Harlequins, capt), M Vunipola (Saracens), T Waldrom (Leicester Tigers), D Wilson (Bath Rugby), T Wood (Northampton Saints), T Youngs (Leicester Tigers)
Backs: B Barritt (Saracens), M Brown (Harlequins), D Care (Harlequins), O Farrell (Saracens), T Flood (Leicester Tigers), A Goode (Saracens), U Monye (Harlequins), C Sharples (Gloucester Rugby), M Tuilagi (Leicester Tigers), B Youngs (Leicester Tigers)