Exiles coach Brian McClennan has included six new faces - three of which are from Hull KR - in his side to face England at the Halliwell Jones Stadium.
Rovers' half-back duo of Michael Dobson and Travis Burns, plus forward Mickey Paea, will appear in the international origin match, which is live on Sky Sports on Friday night, for the first time in their careers.
Zeb Taia (Catalan Dragons), Harrison Hansen (Wigan) and Chris Bailey (London Broncos) are the other debutants selected by McClennan.
Brett Hodgson had already been confirmed as captain of the Exiles, and the full-back will lead out a team that also includes fellow Warrington players Joel Monaghan and Trent Waterhouse for the fixture at their home ground.
"Our preparation has been good, the spirit's excellent, confidence is high and we've got the players to do the job," McClennan said.
"England have got a very tough task ahead of them.
"We really want to produce a style of rugby that does the Exiles brand proud - the kind of rugby the Warrington fans are used to.
"I really hope they come out and support the match in huge numbers - even if they are supporting England."
The former Leeds Rhinos boss would have teamed up Dobson with former Rovers team-mate Blake Green in the half-back positions had the Wigan Warrior not been ruled out through injury.
"It's my first one so I'm looking forward to it," Dobson said. "I was 19th man for the second game last year.
"It's disappointing for Blake but it gives Travis the opportunity to play and hopefully we both do well.
"It is going to help having played at club level together. We've both been injured at different times so we're still a work in progress. We're getting there but we're definitely not the finished article."
St Helens second rower Sia Soliola is the Exiles' only ever-present going into the fourth match against England. Fellow Saint Willie Manu and Widnes full-back Rhys Hanbury were the players to miss out from the 19-man squad.
Exiles team: B Hodgson (Warrington, Aus, capt); J Monaghan (Warrington, Aus), S Menzies (Catalan Dragons, Aus), J Moon (Leeds, Aus), P Richards (Wigan, Ire); T Burns (Hull KR, Aus), M Dobson (Hull KR, Aus); K Leuluai (Leeds, Samoa), H L'Estrange (Bradford, Aus), T Puletua (St Helens, Samoa), S Soliola (St Helens, Samoa), T Waterhouse (Warrington, Aus), H Hansen (Wigan, Samoa).
Interchange: L Hohaia (St Helens, NZ), M Paea (Hull KR, Tonga), Z Taia (Catalan Dragons, Cook Islands), C Bailey (London Broncos, Aus).