England all-rounder Luke Wright admits the one-off fixture against Scotland on Saturday provides a potential banana skin ahead of the five-game NatWest Series with Australia.
The visitors start as heavy favourites at Edinburgh's Citylets Grange, a status they will not enjoy against 50-over world champions Australia.
"It's always a challenge every time you play against sides like Scotland, as we found out in the World Twenty20 against Ireland," said Wright.
"It's a difficult challenge and you have to be at your best to beat them.
"But we have a lot of confidence at the minute and hopefully we can build on that and put in some good performances.
"We've come across quite a few challenges now as a squad and overcome them. Scotland are underdogs and it's always difficult against teams who have nothing to lose. We have to be professional in the way we go about it.
"But confidence-wise the boys are on fire right now and everyone is excited and looking forward to taking on these challenges."
England have a 13-man squad at their disposal as Ryan Sidebottom will definitely not feature due to a hamstring strain.
Sidebottom's latest fitness problem prompted the selectors to call up Yorkshire seamer Ajmal Shahzad, who made his one-day debut during the tour of Bangladesh in March.
Shahzad will only come into contention in the Scottish capital if England opt against playing both spinners, Graeme Swann and Michael Yardy - a formula that proved successful during their triumph at the ICC World Twenty20 in May.
Test regular Ian Bell is set to make his first one-day international appearance for almost 19 months, having re-assessed his approach to batting in the limited-overs format during some net sessions with the ultra-aggressive Wright.
"We've been using the buddy system where you work with different people and Belly and I were together, pairing up in the nets," explained Wright.
"Belly's in great form and it's a bit hard for someone like me to be giving him tips. He's hitting it beautifully so there's not that much to change really."
Scotland fielded four debutants in the defeat to the Netherlands on June 15 but are boosted by the return to fitness of captain Gavin Hamilton, who played a solitary Test for England in 1999, and Durham batsman Kyle Coetzer.
Bowling spearhead Gordon Goudie is, however, a major doubt due to a hamstring strain, while fellow seamer Dewald Nel - who is contracted to Kent - is ruled out by injury.
Hamilton, the 35-year-old former Yorkshire and Durham all-rounder, believes England's class of 2010 is one of their strongest ever.
"It's nice to see an England side performing, having an aura about them, looking like a team of athletes and like a proper international side capable of beating anyone," he said.
"I've played against these guys quite a lot and it's nice to see them on top of the world, literally, in one-day cricket.
"To be playing against them at any stage is good and to be playing against them as world champions is special.
"The way they go about their cricket - their preparation, their platform - means they can transfer to any type of cricket, be it Test match, one-day or Twenty20.
"Absolutely it's the strongest English team I've seen. Everyone knows exactly what their roles are in the side and that stands out."
Scotland (from): G Hamilton (capt), R Berrington, K Coetzer, J Davey, G Drummond, G Goudie, M Haq, R Lyons, D Lockhart (wkt), N McCallum, G Maiden, P Mommsen, M Parker, R Watson
England (from): Andrew Strauss (capt), J Anderson, I Bell, T Bresnan, S Broad, P Collingwood, C Kieswetter (wkt), E Morgan, K Pietersen, A Shahzad, G Swann, L Wright, M Yardy