Juventus dropped their first Serie A points of the season when they were held to a 1-1 draw at Sassuolo.
Leonardo Bonucci struck the winner four minutes from time as Juventus ended Roma's perfect Serie A run with a 3-2 win.
Ten-man Lazio edged Sassuolo 3-2 on Sunday to secure back-to-back Serie A wins for the first time this season.
Napoli survived a late Sassuolo surge to ease the pressure on under-fire boss Rafael Benitez with a 1-0 win.
Juventus defended their place at the top of Serie A with Arturo Vidal on target twice in a 3-0 win over Cesena.
Fiorentina twice hit the woodwork and missed other chances and had to settle for a goalless draw against Sassuolo.