Palermo picked up their first win back in Serie A, but they left it late to beat 10-man Cesena 2-1.
Leonardo Bonucci struck the winner four minutes from time as Juventus ended Roma's perfect Serie A run with a 3-2 win.
Empoli brushed aside fellow strugglers Palermo with a comfortable 3-0 victory.
Filip Djordjevic's hat-trick helped Lazio to a 4-0 victory over Palermo on Monday night.
Juventus defended their place at the top of Serie A with Arturo Vidal on target twice in a 3-0 win over Cesena.
Palermo turned up the heat on Napoli coach Rafael Benitez by snatching a 3-3 draw at the Stadio San Paolo.