Fiorentina captain Manuel Pasqual is expected to be sidelined for four weeks with a muscular injury.
Juventus reasserted their absolute authority in Serie A by humiliating Parma 7-0 in Turin on Sunday.
Gonzalo Higuain fired Napoli into third place in the Serie A table with a 1-0 victory over Fiorentina.
Dinamo Moscow and Salzburg made certain of their places in the Europa League knockout stages, but Inter Milan must wait.
AC Milan suffered a 2-0 home defeat to Palermo in Serie A on Sunday after Sampdoria had earlier seen off Fiorentina 3-1
Sampdoria returned to the Champions League places by beating Fiorentina 3-1 in a frenetic Serie A clash.
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