Napoli missed the chance to move level with second-placed Roma in Serie A as they lost 1-0 at Torino on Sunday.
Fiorentina beat Inter Milan 1-0 to remain clear of Sampdoria in Serie A's final European place.
Liverpool crashed out of the Europa League as Dejan Lovren missed the decisive penalty in a 5-4 shoot-out defeat by Besiktas.
Roberto Soldado's first-half miss was the "key" moment in Spurs' Europa League loss to Fiorentina, said boss Mauricio Pochettino.
Fiorentina beat Spurs 2-0 in their second-leg Europa League round-of-32 tie to progress to the next round 3-1 on aggregate.
A late exchange of goals between Fiorentina and Torino ensured the spoils were shared from a dramatic 1-1 draw.
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