Mark Clattenburg will be the referee for the Olympic final between Brazil and Mexico on Saturday.
World football governing body FIFA made the announcement on Thursday, adding that Stephen Child and Simon Beck will be his two assistants.
It will be the most prestigious match so far of the 37-year-old's refereeing career after he became a FIFA official in 2006.
Clattenburg has officiated in the UEFA Champions League, Euro 2012 qualifying games and last season's Carling Cup final.
David Elleray, chairman of the FA referees' committee, said: "The appointment of Mark Clattenburg to referee the Olympic final with his English assistants Steve Child and Simon Beck is wonderful news and is in keeping with the outstanding achievements of so many people from Great Britain in these Olympics.
"Match officials in all sports make an important contribution to the success of sport and the appointment of the English referee team to the final match should be seen as another 'gold' for Team GB.
"This appointment means that English officials have controlled the World Cup final, Champions League final and Olympic final in the space of two years - a remarkable achievement which reflects well on the health of refereeing in this country.
"As with Howard Webb's World Cup final appointment, this will be an inspiration to our young referees and will mean that the 2012 Olympics will have a lasting legacy in refereeing."