Striker Danny and defender Silvio have been ruled out of Portugal's Euro 2012 play-off games against Bosnia-Herzegovina.
Zenit St Petersburg's Danny has a sprain in his right knee, while Atletico Madrid full-back Silvio has a groin strain.
Silvio said: "I was checked and will be leaving the training camp now due to a groin pain. I do so with regret; no player likes to miss out on such decisive matches as these two but I think we will manage to qualify.
Malaga defender Eliseu has been called up to the squad in place of Silvio, while Danny is replaced by uncapped PAOK winger Vieirinha.
Portugal are looking to avoid missing out on the European Championship for the first time since 1992 and are away in the first-leg on Friday.
Paulo Bento's side finished second in their qualification group after losing to Demark in the final game of the campaign.
Bosnia failed to beat France in their final group game and are looking to reach their first ever major international tournament.