UEFA will begin disciplinary proceedings against the Croatian Football Federation following incidents in their game with Spain.
The Croats have already been fined 80,000 euros (£64,561) on Tuesday for "the setting-off and throwing of fireworks and the improper conduct of supporters (racist chants, racist symbols)" at Thursday's match against Italy.
However, UEFA's latest case against them will centre on the improper conduct of their fans and players during their 1-0 defeat to Spain.
A UEFA statement read: "UEFA has today opened disciplinary proceedings against the Croatian Football Federation (HNS) for the setting-off and throwing of fireworks, and the improper conduct of supporters (display of racist banners and symbols) at the EURO 2012 Group C match against Spain in Gdansk on Monday.
"The disciplinary case also involves the improper conduct of the team (six Croatian players received a yellow card during the match)."
The case will be dealt with on Sunday, June 24.