Arsenal striker Robin van Persie has withdrawn from the Netherlands squad for Wednesday's friendly against Austria due to flu.
The striker played the full 90 minutes and scored twice for the Gunners during their 4-4 Premier League draw against Newcastle at the weekend, but has been deemed unfit for the international game.
The Netherlands Football Union announced on Monday that coach Bert van Marwijk has called up FC Twente forward Luuk de Jong as his replacement.
Van Persie's absence becomes the latest setback for the Oranje after midfielders Rafael van der Vaart of Tottenham and Nigel de Jong of Manchester City also pulled out with injuries.
No replacement was called up for Van der Vaart or De Jong, who was poised to make his international return after being dropped for three matches for a tackle that left Newcastle man Hatem Ben Arfa with a broken leg.
Holland, runners-up to Spain at the World Cup last year, take on Austria in Eindhoven on Wednesday evening.