Wales boss Chris Coleman has decided to rest captain Aaron Ramsey for the pre-World Cup friendly against the Netherlands on June 4.
The Arsenal midfielder, who scored the winner in the FA Cup final last weekend, missed a significant part of the season with a thigh injury before returning to bolster the Gunners' end-of-season push for a top-four place.
"The problem we've got is that he had the same injury problem after Christmas - it was recurring," said Coleman, who has included Real Madrid star Gareth Bale in his 23-man squad.
"We thought he could come with us, but we think he needs a good rest. He is a loss, of course he is, and we are a better team when he is with us, that's obvious.
"But we didn't want to take the risk, neither did Aaron, nor Arsenal, I would have thought. He is getting married also, but that's after the game, so that wouldn't have been a big problem, but we think he needs a bit of a rest so we can get him ready for the start of the season."
Bale, who is set to play in Saturday's UEFA Champions League final against Atletico Madrid, is joined in the squad by five uncapped players including Hull City defender James Chester and Manchester United youngster Tom Lawrence.
Goalkeepers Connor Roberts and Owain Fon Williams and Newcastle defender Paul Dummett will also be hoping to win their first full caps.
Swansea pair Ashley Williams and Ben Davies are rested as they recover from injuries, while West Ham defender James Collins has a calf muscle injury.
Meanwhile, Coleman confirmed that West Brom goalkeeper Boaz Myhill has retired from international football.
"I had a conversation with Boaz in the last camp. He came to me and told me what he was thinking," added Coleman.
"I always say international football is not for everyone and sometimes players who have got families want to spend more time with their families, rather than coming away with us for 10 days here or there doing the extra travelling, and that was Boaz's decision.
"We are disappointed because he has been a valued member of the squad, but he is his own man and he knows what he wants. We've had to accept that and we wish him all the best for the future."
W Hennessey (Crystal Palace), C Roberts (Cheltenham), O F Williams (Tranmere), J Chester (Hull), P Dummett (Newcastle), D Gabbidon (Crystal Palace), D John (Cardiff City), L Nyatanga (Barnsley), A Richards (Swansea), N Taylor (Swansea), J Allen (Liverpool), J Collison (West Ham), E Huws (Manchester City), O Tudor Jones (Hibernian), A King (Leicester), T Lawrence (Manchester United), J Ledley (Crystal Palace), D Vaughan (Sunderland), J Williams (Crystal Palace), G Bale (Real Madrid), S Church (Charlton), J Easter (Millwall), H Robson-Kanu (Reading).