Ashley Cole has been ruled out of England's World Cup qualifier against Ukraine at Wembley on Tuesday evening due to an ankle injury.
The Chelsea left-back suffered the problem in the Blues' UEFA Super Cup defeat by Atletico Madrid in Monaco and missed Friday night's 5-0 romp against Moldova as a result.
Roy Hodgson had hoped that the experienced defender would be available for the Group H contest with Ukraine, but will again turn to Leighton Baines to deputise.
The Everton defender started against Moldova in Chisinau and was rarely tested by a limited home side, before opening his international account with a deflected free-kick in the closing stages.
Centre-back John Terry remains a doubt for the latest meeting with the Euro 2012 co-hosts, who the Three Lions narrowly defeated 1-0 during the group stages of the summer showpiece.
Terry suffered an ankle injury during the victory over Moldova but was forced to play on as England had used all their substitutes, before finally succumbing to the problem shortly before the final whistle.
A statement on the FA's official website read: "John Terry is continuing to receive treatment from the England medical team, having hurt his ankle in the World Cup Qualifier with Moldova on Friday.
"Ashley Cole was assessed by the medical staff on Saturday and will not be available for the qualifier with Ukraine on Tuesday."