Tottenham defender Kyle Walker has been forced to pull out of England's two friendlies because of injury.
It is another blow for Roy Hodgson's set-up ahead of the matches against the Republic of Ireland at Wembley on Wednesday and Sunday's trip to Brazil in Rio de Janeiro.
Manchester United midfielder Tom Cleverley withdrew over the weekend, although there was some good news on Monday with striker Danny Welbeck set to be available despite missing the early training session at St George's Park.
Steven Gerrard (shoulder), Andy Carroll (heel) and Jack Wilshere (ankle) are also missing.
The latest player to withdraw is Walker with the Football Association stating the defender had reported with a fibula injury on Sunday and was released following assessment by their medical staff.
The FA statement read: "The Tottenham Hotspur defender will be unavailable for the upcoming two matches and has returned to his club. There will be no replacement at this time."
Walker hobbled out of Tottenham's friendly against Jamaica in the Bahamas on Thursday following a strong tackle. It means Liverpool's Glen Johnson is the only orthodox right-back remaining in Hodgson's squad.
England do, though, have the option of using Manchester United's Phil Jones in that role, while Everton's Phil Jagielka has also played in that position for his country.
Keeper Joe Hart, Lescott and James Milner all took part in Monday's training, despite having only just returned from Manchester City's end-of-season tour of the United States.
Gary Cahill, Ashley Cole and Frank Lampard, part of the Chelsea squad that faced City in games in St Louis and New York, were also involved.
Uncapped Reading goalkeeper Alex McCarthy was tested in the session at St George's Park, saving shots from fellow goalkeepers Hart and Ben Foster.