Chelsea forward Eden Hazard has been ruled out of the Europa League final against Benfica on Wednesday evening with a hamstring injury.
Rafa Benitez confirmed on Tuesday that the Belgium international had not recovered in time after pulling up in the closing stages of Saturday's 2-1 victory over Aston Villa.
But captain John Terry will be given a chance to prove he has recovered from his ankle problem in time for the Amsterdam ArenA.
Centre-back Terry also limped out of the Villa Park encounter at the weekend but a final decision on his fitness will not be made until nearer the kick-off.
"Hazard will not be available," Benitez said. "John still we will try and see how he feels. We will try to see tomorrow."
Terry missed last season's UEFA Champions League final win in Munich through suspension and his injury means he faces further continental heartache.
"Now it's not easy for him," Benitez added. "It's not the best injury, but we will see today, we will see tomorrow with the medical staff and after we will decide."
The 32-year-old former England captain's place in the side was already doubtful in a competitive position in central defence, with David Luiz, Gary Cahill and Branislav Ivanovic the Blues' other options.
The loss of Belgian playmaker Hazard will be more keenly felt by the Blues as they go in search of back-to-back European trophies.
Coupled with the concern over John Obi Mikel, who has a hip problem, it may mean Frank Lampard is included, in all likelihood as captain.
Lampard, who became the club's record goalscorer by netting twice in the win at Villa Park, could be deployed in a deep midfield role alongside Luiz, with Ramires pushed further forward alongside Juan Mata and Oscar.
Lampard's future has been shrouded in doubt for much of the season.
The England midfielder, who will be 35 in June, is out of contract at the end of the season, with talks on his fate likely to take place after Sunday's 69th and final match of the season at home to Everton.
Lampard said: "I don't know what the future exactly holds, but it doesn't matter tomorrow night. It's about winning the game."
Chelsea travelled to Amsterdam with a 25-man squad, with left-back Ryan Bertrand (knee) a doubt, but midfielder Oriol Romeu is in contention following his long-term absence with a knee problem.
Demba Ba also travelled, but the striker is ineligible after playing for former club Newcastle earlier in the competition.