FC Twente suffered their first defeat to Roda JC while Ajax went top of the table and AZ thumped Vitesse 4-0.
Reigning champions Ajax took pole position on Saturday evening after snatching a 3-2 victory at Heracles Almelo.
Siem de Jong bagged a double for the Godenzonen and a late goal from Heracles Glynor Plet was not enough to overthrow the visitors.
Twente's unbeaten start to the season came to an end as they let slip a lead to lose 2-1 at Roda JC.
Marc Janko put the 2010 champions ahead in the 35th minute but Ruud Vormer had the hosts level within a minute and Mitchell Donald completed the turnaround with the second goal two minutes into the second half.
Twente's defeat allowed AZ Alkmaar to take the second spot after they claimed an emphatic 4-0 victory over Vitesse.
Pontus Wernbloom grabbed a first-half double for the hosts to inspire the rout before goals from Rasmus Elm and Roy Beerens put the result beyond doubt.
Promoted RKC Waalwijk sprung a surprise at ADO Den Haag as they secured a 1-0 victory to move into fourth place.
Young striker Rick ten Voorde was behind the road triumph after he knocked home the winner in the 33rd minute.
Soufian El Hassnaoui was on target for De Graafschap as they secured a 1-0 win at home to NEC Nijmegen.