PSV Eindhoven lost again in the Eredivisie as they went down 3-1 at Ajax on Saturday night, while FC Twente beat Den Haag.
PSV crashed to their second consecutive defeat in the league, their defeat away to the reigning Dutch champions allowing Twente to take over at the top of the table.
Dick Advocaat's PSV saw an eight-game winning run ended by surprise packets Vitesse Arnhem last weekend and a trip to Amsterdam produced a further blow to their ambitions as they slumped 3-1.
Jeremain Lens had sent PSV into the break level as he fired a 36th-minute equaliser after Siem De Jong opened the scoring seven minutes earlier.
The score remained locked until Danny Hoesen broke through 18 minutes from the end and Viktor Fischer sealed Ajax's win - their fourth on the spin - in the shadows of full-time.
PSV's slip allowed Twente to jump a point clear at the top with a 2-0 win over 10-man ADO Den Haag.
Tjaronn Chery saw red on 49 minutes and Twente's goals followed thereafter with Dusan Tadic (55) and Dmitri Bulykin (83) on target.
Fifth-placed Feyenoord remained on the coat-tails of the top clubs after they were also 2-0 winners over RKC Waalwijk.
Graziano Pelle (45, 57) grabbed both goals to leave the Rotterdammers four points off the top.
Willem II snatched a second league win 3-1 over Heerenveen although it was not enough to lift them off the foot of the table.
They needed late goals from Hans Mulder (79) and Aurelien Joachim (90) after Alfred Finnbogason (70) had cancelled out Virgil Misidjan's 18th-minute opener.
A forgettable night for Heerenveen also saw Jeffrey Gouweleeuw dismissed on 84 minutes.
PEC Zwolle and VVV-Venlo played out a goalless draw on Friday evening which did little for either team's cause.
The promoted hosts had the better of the chances but could not find a way past VVV goalkeeper Niki Maenpaa, who produced a fine reflex save on the stroke of half-time to keep out Jesper Drost's deflected shot and also denied Denni Avdic in stoppage time.
The result lifted VVV one place to 16th in the standings - one position and still two points worse off than their opponents.