Relegation threatened Heracles Almelo will have to scrap for their lives without captain Rob Maas.
The veteran midfielder damaged knee ligaments against Twente on Sunday and has been informed he will play no further part in their campaign.
The news has come as a huge blow to Heracles coach Ruud Brood.
"The fact that Rob cannot play with us in the final stage of the competition is a blow for us," Brood told Voetbal International.
"In our battle to stay in the Eredivisie he is of great worth to us."