PSV Eindhoven missed the chance to extend their lead at the top of the table after being held to a goalless draw at struggling Excelsior.
Ronald Koeman's men were far from their best as they failed to see off their struggling opponents.
AZ Alkmaar failed to capitalise on PSV's draw as they were held to a goalless draw at home to Feyenoord.
Ajax leapfrogged FC Twente into third spot in the table with a thumping 4-1 win as Klaas Jan Huntelaar helped himself to a hat-trick.
Twente made a dream start with Blaise N`Kufo firing them in front after just ten minutes but Huntelaar drew Ajax level on 21 minutes.
The Dutch international gave Ajax the lead on the half hour mark and Ryan Babel added a third goal right on the stroke of half-time.
Huntelaar completed his treble with 15 minutes to go to ensure the win for Ajax.
Fred Benson's late goal earned Vitesse Arnhem a 2-2 draw with basement boys ADO Den Haag.
Santi Kolk opened the scoring for Den Haag on 11 minutes, but the lead proved short-lived with Mads Junker drawing Vitesse level four minutes later.
Vitesse suffered a blow on 37 minutes when Giovanny Espinoza was sent-off for a professional foul and from the resulting penalty Kolk restored Den Haag's lead.
Den Haag were reduced to ten men in the final minute when Eljero Elia was sent-off and there was still time for Benson to pop up with the equaliser in the dying seconds.