Denny Landzaat and Sander Boschker have been dropped from the Holland squad for Euro 2008.
FC Twente goalkeeper Boschker had been called into the provisional squad for the first time, while Feyenoord midfielder Landzaat was a member of the 2006 World Cup squad.
It means Holland coach Marco van Basten still has to trim one player from his squad before Wednesday's deadline for submitting the final 23-man squad.
Van Basten admitted earlier this week the last player to be dropped would be one of three defenders: Andre Ooijer, Khalid Boulahrouz or Mario Melchiot.
Wigan defender Melchiot missed training this week because of injury and will have to show on Monday and Tuesday that he has recovered sufficiently.
"We want to see how Melchiot is doing. Boulahrouz made a good impression and Ooijer is fit," said Van Basten after Saturday's 3-0 win against Ukraine