The Lazio defender injured himself playing against Parma in the 1-0 defeat at Ennio Tardini.
Italian international coach Giovanni Trapattoni may call-up Verona central defender Marco Zanchi to replace Negri, although another option is Christian Pannucci at Roma.
Also, Fiorentina's Alessandro Pierini may have the chance to impress as it is thought that Trapattoni may also hand him a place in the squad.