Reggina stunned champions Milan 2-1 to ensure their safety and Siena left Modena in real trouble after a 3-1 win.
David Di Michele gave Reggina the ideal start over Milan with the opener after just six minutes and Francesco Cozza doubled the lead just before the half hour.
Andrei Shevchenko pulled a goal back for Milan on 50 minutes, but Reggina held on to secure their place in Serie A for next season.
Siena ensured their survival with a superb victory at Modena.
Rodrigo Taddei netted the opener after 40 minutes and Modena suffered a major blow when Mauro Mayer was sent off just before the hour mark.
Siena made their numerical advantage count with Taddei grabbing his second nine minutes from time.
Modena gave themselves hope with Massimo Marazzina pulling a goal back four minutes later, but Enrico Chiesa ended their hopes with Siena's third in the dying moments.
Perugia boosted their survival hopes with an impressive 3-1 win at second-placed Roma.
Antonio Cassano gave Roma an early lead after just 11 minutes, but Perugia fought back with Jose Ze Maria drawing them level seven minutes later.
The Brazilian then gave the visitors a shock lead with his second from the penalty spot on 23 minutes and former Middlesbrough striker Fabrizio Ravanelli added a third seven minutes from time
Ancona recorded only their second win of the season with a 2-1 victory over struggling Empoli.
Mauro Milanese gave Ancona the lead after 18 minutes, but Ighli Vannucchi levelled matters for Empoli on 68 minutes.
Vincenzo Sommese grabbed the winner for Ancona firing home from the penalty spot on 73 minutes.
Internazionale leapfrogged Parma into fourth spot with a win at the Giuseppe Meazza
Brazilian hit-man Adriano came back to haunt old club with a brilliant free kick on 62 minutes and Parma's hopes of getting back into the contest went up in smoke with Manuele Blasi seeing red for a second bookable offence after 78 minutes.
Brescia put a huge dent in Lazio's Champions League hopes with an impressive 2-1 win.
The home side left it late with Stefano Mauri breaking the deadlock ten minutes from time and Roberto Baggio made the game safe with the second two minutes from time.
Brazilian defender Cesar reduced the arrears in the final minute for Lazio, but Brescia held on for the win.
Udinese secured their place in next season's Uefa Cup after a 1-1 draw with Chievo at Friuli.
Federico Cossato grabbed the opener for Chievo on 31 minutes, but Vincenzo Iaquinta drew Udinese level right on the stroke of half time.
Juventus got back to winning ways after two successive defeats with a 2-0 success over Sampdoria at the Stadio Delle Alpi.
Nicola Legrottaglie fired Juve in front on 35 minutes with a rare goal and Stephan Appiah doubled the advantage three minutes before the break.
Javier Chevanton's last gasp goal earned Lecce a 1-1 draw at Bologna at the Stadio Dall'Ara.
Albanian international Igli Tare looked to have fired the winner for Bologna after 32 minutes, but Chevanton popped up to snatch a point for Lecce in stoppage time.
