Monday 16 March 2015 23:21, UK
Roma fell to their first defeat in Serie A since November 1 as they suffered a surprise 2-0 home defeat to Sampdoria.
With Lazio winning at Torino earlier in the evening to move within a point of their city rivals, Roma could ill afford a slip but endured a miserable evening as they ended the match with 10 men.
Lorenzo De Silvestri and Luis Muriel grabbed the goals for the Blucerchiati, who weathered a first-half storm before striking efficiently in the second half to boost their own hopes of qualifying for Europe next season.
Roma's sorry evening ended on an even worse note with Seydou Keita shown a red card late on as the Giallorossi also helped Juventus get one hand on a fourth straight Serie A title, thanks to their 14-point advantage with 11 games remaining.
Lazio won 2-0 at Torino courtesy of two goals in the space of seven minutes from Felipe Anderson late on.
The victory gives them a three-point cushion over Napoli in the hunt for the third Champions League berth, but they may have their sights set higher.
Fiorentina came from behind to pile the misery on Filippo Inzaghi and his AC Milan team with a 2-1 win at the Stadio Artemio Franchi.
Mattia Destro put the visitors in front against the run of play early in the second half, but Fiorentina fought back with goals from Gonzalo Rodriguez and Joaquin inside the final seven minutes earning them all three points.
As a result, Vincenzo Montella's men now trail fourth-placed Napoli by just one point with Lazio in third just four ahead.
Milan, meanwhile, remain in 10th place, 10 points behind the Viola with 11 games left to salvage their season.
