Serie A players will go on strike for one weekend following a breakdown in negotiations between the Lega Calcio and the players' union.
The two parties were trying to agree on a new collective bargaining agreement but the players' union walked out on talks on Tuesday.
A strike was also announced in September but was postponed to allow more talks, with a deadline of November 30 given to reach a resolution to the dispute.
A players' strike is now highly likely for the round 16 fixtures next month.
"The strike has been called for December 11 and 12," Leonardo Grosso, vice-president of the players' union, Associazione Calciatore Italiana, said after the meeting.
"We are really disappointed because we were open to negotiate and to find common ground, but no agreement has been reached."
The union refuses to accept a proposal that clubs can force unwanted players to train away from the first team or be forced to accept a transfer.
Italian Football Association president Giancarlo Abete joined the talks but failed to find a breakthrough.
Abete will now accompany the sides to a hearing at the Italian Olympic Committee on Wednesday, when they will again attempt to head off strike action.