Villarreal defender Rodolfo Arruabarrena has been ruled out for up to three weeks with a thigh strain.
The left back sustained the injury after just 13 minutes of Sunday's goalless home draw with Espanyol.
Subsequent tests have revealed a small tear in the 31-year-old's left thigh and he will now be sidelined for between two and three weeks.
Arruabarrena's absence will present summer signing from Celta Vigo, Jose Enrique, with the chance to make his debut for the club.
Villarreal currently lie tenth in La Liga having taken eight points from their opening six games.