A weakened Real Madrid side were held to a 1-1 draw at third division side Ecija in the Copa del Rey.
The Santiago Bernabeu giants made the trip to Andalucia for their fourth round, first leg tie on Wednesday evening, as Real boss Fabio Capello gave David Beckham only his second start in eight matches.
Instead, Italian striker Antonio Cassano (pictured) found the scoresheet for Real on 66 minutes before Ecija struck a leveller through striker Nolito nine minutes from time.
Primera Liga champions Barcelona got a brace from Eidur Gudjohnsen (62, 76) to win 2-1 at third division club Badalona.
Pedro Tarradellas struck a late penalty for the home side ahead of the second leg at Camp Nou.
A brace from Miguel Angel Angulo gave Valencia a 2-1 first-leg win at another third division side in Portuense, who initially took the lead through Ocana.
A first-half strike from Blanco Kepa gave Sevilla a 1-0 win at Gimnastica Segoviana, while a Fabian Estoyanoff penalty (51) gave Deportivo La Coruna a 1-0 home win over Primera Liga rivals Racing Santander.
Pena Sport held Osasuna to goalless home draw, while holders Espanyol left it late to claim a 1-1 away draw at third division side Rayo Vallecano.
A Diego Torres penalty (42) gave Vallecano the lead before Luis Garcia equalised with a spot kick of his own in the last minute.
A strike from Alvaro Anton (69) gave Real Valladolid a 1-0 win over Gimnastic de Tarragona, while ten-man Hercules drew 1-1 with Real Zaragoza.
Juanlu (16) gave Hercules the lead before they were reduced to ten men when Redondo (66) was sent off.
Former Portsmouth man Andres D'Alessandro equalised two minutes later for Zaragoza.
Segunda Liga club Malaga took a firm grip on their fourth-round tie after a 4-1 first-leg home demolition of struggling Real Sociedad.
After Inigo Diaz de Cerio (8) gave Sociedad the lead, the Basque club lost Alvaro Novo to a second yellow card offence.
That allowed the Andalucians to open the floodgates, as goals from Anderson Silva (45), Ernesto (61), Vicente Valcarce (68) and Pedro Pinazo Perico (81) completed the rout for Malaga.
A Pablo Redondo penalty (74) and a strike from Sergio Pachon (76) gave Getafe a 2-0 win at Xerez, while Villarreal got goals from Nihat Kahveci (12) and Diego Forlan (24) to win 2-0 at Castellon.
Recreativo de Huelva held Andalucian rivals Real Betis to a goalless first-leg draw at the Nuevo Colombino, while Athletic Bilbao drew 1-1 at home with Real Mallorca.
Igor Gabilondo (12) gave Athletic the lead before The Islanders earned a first-leg draw at San Mames through Maxi Lopez (50).
On Tuesday evening, a goal from striker Nino gave Levante a 1-0 win at Atletico Madrid, while second division Alaves were held to a goalless home draw by 10-man Celta Vigo, who had Lopez Ruano Angel sent off.
The second leg ties are due to take place on November 8.