Gazelec Ajaccio provided the shock result in the Coupe de France last 32 with a 1-0 win over Toulouse.
Meanwhile Ligue 1 leaders Paris Saint-Germain spared new coach Carlo Ancelotti from humiliation with a stoppage time winner against minnows Locmine Saint-Colomban.
There were also wins for Lyon in a local derby and Evian TG, who got through in a penalty shoot-out.
Corsican side Gazelec Ajaccio, promoted to the third tier Championnat National last season, scored the only goal of the game against Toulouse early in the second half to claim a famous win.
Mickael Colloredo found the back of the net after being set up by Anthony Colinet.
Louis Poggi hit the post but Toulouse's Mohamed Fofana was denied by a goal-line clearance and was then ruled offside when turning home Wissam Ben Yedder's shot late on.
It was the second successive year Le Tefece had been eliminated at this stage of the competition by a team from the third tier, while Le Gaz maintained their three-year unbeaten record at the Stade Ange Casanova.
Diego Lugano's 93rd-minute header gave Paris Saint-Germain a 2-1 victory over fifth-tier Saint-Colomban Locmine in Ancelotti's first official game in charge.
Javier Pastore had given the visitors an early second half lead when he slid the ball home from a narrow angle.
However, the hosts restored parity when Maiga calmly wrong-footed Salvatore Sirigu from the penalty spot after Mamadou Sakho had fouled Fabrice Peru.
And with the game at Lorient's Stade du Moustoir heading into extra time, Uruguay captain Lugano stunned the home crowd by converting Nene's cross to win the tie.
Lyon had no such problems as they beat Lyon Duchere 3-1 in a local derby.
Lisandro Lopez settled the visitors' nerves early on with a fourth-minute goal en route to a first-half hat-trick, Rafik Bouderbal striking a consolation for the home side reason shortly before the interval.
Evian TG were made to work hard by Ligue 2 Metz, eventually going through on penalties after coming from two goals down in a dramatic extra-time period.
The game was goalless at 90 minutes but Mahamane Traore's double looked to have sent the second-tier side into the last 16.
But Cedric Cambon and Yannick Sagbo hauled the Ligue 1 side level and they prevailed in the shootout after Oumar Pouye blazed Metz's third penalty over.
Montpellier hammered minnows Prix-les-Mezieres 4-0 in the day's late kick-off to move safely through to the next round.
Ligue 2 side Istres won 4-1 at non-league opponents Thiers thanks to a hat-trick from Nassim Akrour.