Ten clubs are considering a breakaway from the Scottish Football League and have proposed the formation of an SPL2.
The clubs involved make up the current First Division, with the exception of promoted Partick Thistle and relegated Airdrie United.
But Dundee, who appear certain to be relegated from the SPL, and Second Division winners Queen of the South have both signed up to the changes.
The development comes after a meeting at New Douglas Park on Thursday, when the clubs decided they would try and force a restructuring of Scottish football.
Hamilton chairman Les Gray confirmed the clubs had grown frustrated with the Scottish Football League board and the rebel group hope the SPL will meet them to discuss a two-tier structure.
He said: "We have always said we would prefer a 42-club solution. If they are unable to do that, we can't sit on our hands and see what happens.
"We have given them (SPL) an alternative and that option includes a second tier, a Championship. That's all there is to it, there is nothing sinister behind this.
"I have spoken to the SPL chairman over this and I have always felt there was some warmth towards the idea of a second tier, but it has never got over the line.
"We are going at it from the angle that this is the last chance to do something for next season and there is a will to do it.
"There are clubs in danger, there are clubs having to consider part-time, and there are jobs in danger. We need to try to get this through so that we can create extra wealth for that second tier."
The SPL confirmed they would discuss the proposal at a meeting of the clubs on Tuesday.