Crawley Town will be looking to maintain their lead at the top of the League Two table while second-placed Southend are hoping to return to winning ways.
Crawley's 3-0 win over Burton Albion last weekend was their third on the bounce while Crewe come into the Gresty Road contest on the back of a 1-1 draw at Northampton.
Southend, who lost to Oldham in their FA Cup replay in midweek, can move level on points with Crawley, albeit for 24 hours with success over lowly Bradford on Friday.
Cheltenham are the division's form side and go in search of their fifth consecutive league success against a Barnet side who are six points clear of the drop zone.
Shrewsbury lost to Aldershot last weekend and will be confident of securing the three points against Port Vale. The Valiants have won one of their last five in the league.
Fifth-placed Burton Albion take on rock-bottom Dagenham & Redbridge. The Brewers' three-game winning run was snapped by Crawley while the Daggers have lost nine straight in league action.
Swindon Town, who are unbeaten in nine league outings, take on Morecambe at the County Ground. The Shrimps have dropped out of the play-off picture after going four without success.
Gillingham occupy sixth place despite drawing their last two and they tackle a Bristol Rovers side who have won just one of their last six in League Two.
Plymouth are level with lowly Dagenham at the wrong end of the table, but the Pilgrims could close the gap on the Bantams with a home victory against 21st-placed Hereford.
AFC Wimbledon's slide down the table continued after a 2-0 defeat by Accrington last weekend. The former non-leaguers travel to South Yorkshire to play Rotherham, who have won three of their last four.
Accrington are moving in the right direction on the back of a five-game unbeaten run, something they will be looking to extend as Torquay United make the lengthy trip up north.
Shrewsbury's game with Macclesfield and Oxford's contest with Northampton at the Kassam Stadium conclude the weekend's League Two programme.