Grimsby were relegated from League Two, as Morecambe and Dagenham sealed the final play-off places.
Grimsby needed to win before even thinking about other results, but they were well beaten 3-0 at Burton.
Greg Pearson scored after 10 minutes for The Brewers before two goals from Shaun Harrad either side of the break confirmed Grimsby's 99-year tenure in the league had come to an end.
Barnet and Cheltenham were both left knowing they would be in League Two again next year.
Barnet ended up winning 1-0 against already promoted Rochdale with Albert Jarrett scoring in the last minute.
Cheltenham drew 1-1 with Accrington with Chris Turner equalising after Josh Low had put the Robins ahead.
At the other end of the table, six teams were battling for the final two play-off places - but it was Morecambe and Dagenham who managed to cling on with wins.
Morecambe finish fourth after they beat Aldershot 1-0 with Garry Hunter grabbing the winner just 18 minutes from time.
The win takes the Shrimps above Aldershot, and also Rotherham - who were well beaten 3-0 at Hereford, with Mathieu Manset, Darren Jones and Marc Pugh scoring.
Dagenham & Redbridge secured seventh place with a 2-0 win at relegated Darlington.
A goal in each half from Jon Nurse and Josh Scott did the job for John Still's men.
Bury missed out after drawing 1-1 at Northampton - who were another of the four looking to hijack the play-off places.
Bury had gone ahead at the Sixfields with Ryan Lowe scoring on the stroke of half-time, but Ade Akinfenwa levelled for the home side in the final minute.
Port Vale also still harboured play-off hopes - but they were held to a 1-1 draw by Shrewsbury.
Kris Bright put the Shrews ahead, but Marc Richards' second-half penalty secured a draw for Port Vale.
Chesterfield were the final side who had play-off hopes but they bid farewell to their Saltergate home with a 2-1 win over promoted Bournemouth.
Bournemouth took the lead just before the break with Drew Talbot putting through his own net.
The equaliser came ten minutes from time when Jack Lester scored before Derek Niven fired home in the final minute.
Bradford finished 14th, after they finished their campaign with a 1-0 win at Crewe thanks to Ryan Kendall's goal just after the hour.
Champions Notts County signed off from League Two life with a goalless draw at Torquay.
Finally, Lincoln played out a 0-0 draw with Macclesfield at Sincil Bank - despite having Davide Somma dismissed.