MK Dons' first defeat of the season allowed Sheffield United to move into top spot in League One ahead of Charlton Athletic on goal difference.
Chesterfield and Rochdale recorded first wins of the campaign at the bottom of the table, while Huddersfield Town and Hartlepool United remain unbeaten.
Gary Madine's second-half brace maintained Sheffield Wednesday's 100 per cent home record and inflicted a first defeat on MK Dons following a 3-1 win at Hillsborough.
Sheffield United were unable to take full advantage of playing against ten men for over an hour after requiring a 72nd minute equaliser from Ched Evans to claim a 1-1 draw at Scunthorpe United.
Charlton are level on points with the Blades after Bradley Wright-Phillips and Dale Stephens scored in a 2-0 home win over Exeter City, who had Daniel Nardiello sent off after just 19 minutes.
Huddersfield Town stretched their lengthy unbeaten run in League One as goals from Gary Roberts and Scott Arfield secured a 2-0 home win over Tranmere Rovers.
Cristian Montano notched a 65th minute winner as Notts County, fresh from playing Juventus on Thursday, overcame Walsall 2-1 at Meadow Lane.
Brentford continue to impress under Uwe Rosler as Kevin O'Connor's first-half penalty clinched a 1-0 win away to Wycombe Wanderers.
Hartlepool United maintained their unbeaten start to the season as first-half goals from Gary Liddle and Adam Boyd secured a 2-1 win at Carlisle United.
Chesterfield gained a first victory since winning promotion to League One as new loan signing Leon Clarke was among the scorers in a 3-0 success away to Bournemouth.
Rochdale also picked up three points for the first time this term courtesy of a thrilling 4-2 triumph away to local rivals Bury as Nicky Adams scored and was sent off against his former club.
Leyton Orient continue to await a first win of the season and had Jimmy Smith's last minute equaliser to thank for a 1-1 draw at Colchester United.
Veteran striker Shefki Kuqi was on target again as Oldham Athletic recovered from conceding an early goal to draw 1-1 at home with promoted Stevenage.