Leyton Orient have joined Tottenham Hotspur in launching legal action against West Ham United's proposed move to the Olympic Stadium.
Spurs applied for a judicial review against Newham Borough Council and Orient have now followed the same path.
Orient have also confirmed they will be making subsequent judicial review applications against the Government, Premier League, the Minister of Sport and the Mayor of London.
The club's owner and chairman, Barry Hearn, revealed that the point of law behind their action against Newham Borough Council is their £40million loan to the company which will be set-up to run the stadium after the Olympics and who will rent the venue to West Ham.
Hearn told Sky Sports News: "The whole plan is that we do not want West Ham in the Olympic Stadium, it is black and white, we think they are on our patch.
Big forces of evil
"We think various parties have acted unlawfully and illegally and they need to be called to task. We have started today with the official announcement we have submitted for judicial review an application request against Newham Borough Council.
"The £40million has been illegal and unlawful, it is not what a council should be doing, they do not have the powers and have acted beyond their powers to give this £40million loan.
"It represents a state subsidy, a subsidy of a Premier League football club which is against European and British competition laws, we are very solid in our case and we will pursue this to the bitter end.
"We follow this with judicial applications against the Government, the Minister of Sport, the Mayor of London, and we take action against the Premier League for breaching their own rules as well.
"This is an all-encompassing charge by Leyton Orient, a battle by the little man against the big forces of evil if you like, this represents a challenge to our future and we have no choice but to fight our corner, and we believe we have right on our side."
The Olympic Park Legacy Company (OPLC) backed West Ham's bid to use the stadium after the games in February.