Kevin Davies will miss Preston's next three games after the appeal against his suspension was unsuccessful.
The veteran striker was dismissed in North End's last game against Coventry, a match he scored in, for a high-boot challenge on Cyrus Christie.
North End had hoped to get that ruling overturned but boss Simon Grayson revealed on Wednesday that their attempts had been frivolous.
"We appealed against Kevin's red card and got the report back saying that our appeal had been dismissed and the ban has been upheld," he said.
"We felt we had a shout and if you don't try, you don't succeed.
"Kevin played well at the weekend, but we have plenty of striking options, whatever formation we choose to play; Joe Garner, Iain Hume, Paul Gallagher and Stuart Beavon. We are disappointed to lose him for three games, but it gives someone else a shout."
Davies is now due to miss Friday's FA Cup clash against Nottingham Forest and the League One games against Bradford and Notts County.