Michael O'Connor and Garry Thompson were notable absentees as Scunthorpe won 3-1 at Winterton Rangers on Tuesday night.
The pair missed Saturday's opening pre-season match at Bottesford Town and were again not risked as goals from Andy Barcham, Bobby Grant and Chris Dagnall saw the Iron home.
More new players are expected to arrive at Glanford Park this summer, according to goalkeeping coach Neil Cutler.
"The gaffer is always looking and there are one or two positions we would still like to fill," said Cutler.
"Nothing will probably happen in time for the next friendly at Halifax on Saturday, but maybe before the start of the season. You should never say never."