Paul Simpson has pledged to show negative Preston players the Deepdale exit.
The Preston manager has questioned some of his players' team spirit in the wake of a crushing 3-0 defeat to Colchester on Saturday.
Preston remain the only club in the Football League not to have scored this season and Simpson insists he will now try to remove players with a 'hidden agenda'.
"We have a relationship at the club where people can speak their mind and say where it is going wrong," said Simpson.
"But I want people with positive opinions not those who make things personal and have a hidden agenda.
"We've got a nucleus of a very, very strong squad who all want the same things, but unfortunately there are other players who are trying to cause problems.
"What we have to do as a football club is get those people out of the building in the best way that we possibly can given the guidelines set by the PFA.
"I am actively looking into that. Then we can work with those who want to be here and get a tight knit unit who are together and want to work forwards.
"We've got to make sure that this is the lowest point we get to. This is the most disappointed I've felt in a long, long time."
Meanwhile, Colchester boss Geraint Williams has admitted his delight with the Teddy Sheringham inspired victory and has praised the veteran striker.
"To be fair it doesn't get any better than that," said Jones. "We worked hard and when our chances came we took them clinically.
"Teddy is class. He always has been and he always will be and he'll never lose that."