League leaders Swindon can assure themselves of a top-seven finish on Friday, while Hereford meet Plymouth in a crucial clash at the bottom.
Elsewhere, Torquay and Crawley will be looking to cement their standing inside the automatic promotion places and Cheltenham will want to snap out of an alarming slump.
Swindon head into the Bank Holiday weekend knowing that a point away at Morecambe will assure them of a play-off spot at worst, while victory on the road will strengthen their grip on top spot.
Second-placed Torquay know they have played a game more than all of those around them, and that makes it all the more important that they pick up a positive result at home to Accrington.
Crawley have embarked on an eight-match unbeaten run at just the right time, but play host to a Crewe side on Friday that has gone 10 games without defeat to surge into the play-off picture.
Shrewsbury have their sights set on a return to the top three, and will fancy their chances of picking up maximum points away at struggling Macclesfield - who have gone 17 without a win.
Southend are also still very much in the race for a top-three finish and will head to Bradford looking to keep their promotion push on track.
Oxford sit sixth and will want to respond positively to last weekend's 2-1 reversal against Morecambe when they go to Northampton.
Cheltenham have seen five defeats in their last six outings spoil much of their good work this season and they could slip out of the top seven if they fail to address their wobble at home to lowly Barnet.
Gillingham are among those knocking on the play-off door and will be aiming to close on the promotion chasers when they make the trip to Bristol Rovers.
AFC Wimbledon and Rotherham have little to aim at this season, but will be playing for pride when they go head-to-head on Friday.
Aldershot also appear to have too much to do in order to enter the play-off lottery, but can keep their fading dreams alive with victory over troubled Port Vale.
Dagenham & Redbridge and Burton Albion have managed to haul themselves clear of trouble, but they cannot afford to relax heading down the final straight.
Hereford prop up the division with just six games of their season remaining, but can close to within touching distance of safety and drag a rival back into the mix when they entertain fellow strugglers Plymouth.