Leicester City were the big winners in the Championship as Cardiff City drew at home and Sheffield Wednesday shocked Hull.
Barnsley stunned Yorkshire rivals Leeds United, Middlesbrough and Crystal Palace both suffered defeat in their bids for promotion, while Blackpool's first game since Michael Appleton's departure also ended with a loss.
Birmingham boss Lee Clark had to settle for a point on his return to former club Huddersfield Town, while there were wins for Brighton & Hove Albion and Nottingham Forest, as Millwall drew at Bolton Wanderers.
Leaders Cardiff were frustrated in front of their home fans after being held to a 0-0 draw by lowly Ipswich Town.
January signing Chris Wood scored a first-half hat-trick as Leicester produced a ruthless performance to hammer struggling Bristol City 4-0 at Ashton Gate and dump Derek McInnes' side to the foot of the table.
In Saturday's late game, struggling Sheffield Wednesday caused upset at second-placed Hull City with a 3-1 victory as Reda Johnson's opener, an Eldin Jakupovic own-goal and Michail Antonio's strike sealed the Owls' shock win.
Matej Vydra scored a goal in each half as Watford recorded a 2-1 win at play-off rivals Middlesbrough, who replied in stoppage-time through Scott McDonald.
Managerless Barnsley bounced back to form in impressive style as a second-half brace from Chris Dagnall secured a 2-0 win over Yorkshire rivals Leeds at Oakwell.
Elliott Ward marked his return to the City Ground on loan with an 83rd minute winner as Nottingham Forest overcame Peterborough United 2-1 after the visitors had drawn level through former Forest loanee Scott Wootton.
It was a tale of two penalties at the Reebok Stadium as Keith Andrews' 34th minute spot-kick earned Bolton a 1-1 draw at home to Millwall after the visitors had gone in front on nine minutes through Andy Keogh.
Crystal Palace's promotion bid was checked at Turf Moor as Junior Stanislas' 81st minute goal earned Burnley a 1-0 win.
Appleton's departure for Blackburn Rovers on Friday night left Blackpool without a permanent manager for the trip to Charlton Athletic and goals from Johnnie Jackson and Scott Wagstaff gave the home side a 2-1 win.
First-half goals from Ashley Barnes and Andrea Orlandi kept Brighton in the hunt for a play-off spot following a 2-1 home success over Derby County.
Huddersfield substitute Adam Hammill scored a last-gasp leveller to cancel out Callum Reilly's opener for Birmingham as their encounter ended in a 1-1 draw.