Allan McGregor, Barry Robson and Stephen Pearson have become the latest players to withdraw form the Scotland squad.
Rangers trio Barry Ferguson, Lee McCulloch and Christian Dailly have already pulled out of George Burley's squad for Wednesday's friendly with Croatia.
McGregor has joined his Gers team-mates in missing the game after the goalkeeper was taken out of Burley's plans on Monday lunchtime.
Celtic's Robson has also joined the list of absentees, although the midfielder's injury has not been specified.
Derby County midfielder Pearson will also be absent in midweek after being troubled by a hernia problem in recent weeks.
Pearson did play the final 30 minutes of Derby's defeat to Middlesbrough on Saturday, but is not fit enough for international duty.
Burley, who will be taking charge of Scotland for the first time against Croatia, has called up Cardiff City midfielder Gavin Rae as a replacement.