Sunderland's nightmare start to the season continued with defeat to The Football League's bottom club Bury in the Carling Cup, while Burnley, Cardiff City, Coventry City and Crystal Palace were also the victims of a first round shock.
Burnley slipped up at home to Hartlepool United, while fellow League Two side Barnet accounted for Cardiff, league new boys Hereford United thumped Coventry and Notts County rocked Palace.
Bury, like Sunderland, have not picked up a point this term and prop up the other 91 league clubs, but they stunned the Championship side with a 2-0 victory at Gigg Lane.
Niall Quinn's turbulent start to management continued when recent signing Arnau Riera was sent off after just four minutes.
The breakthrough arrived on 82 minutes when John Fitzgerald pounced, only to then pick up a second yellow card for his celebration.
The Shakers did not wilt, though, and wrapped up a famous win when Andy Bishop scored a second goal two minutes from time.
A Stuart Fleetwood hat-trick helped Hereford to cause an upset as they beat Coventry 3-1 at Edgar Street.
Fleetwood scored after just a minute and added his second goal 12 minutes into the second half.
Dele Adebola quickly pulled one back for the visitors, but Fleetwood completed his treble on 64 minutes to send Hereford into the second round.
Joel Porter's 78th minute penalty, after Wayne Thomas had been sent off, was enough for Hartlepool to record a surprise 1-0 success at Burnley.
A goal in each half from Tresor Kandol helped Barnet to spring a surprise in South Wales as Paul Fairclough's men beat Cardiff 2-0.
Palace appeared to be in control at home to Notts County when Michael Hughes scored after 17 minutes.
Lawrie Dudfield equalised six minutes later and Daniel Martin was on target on 84 minutes to send The Magpies into the next round.
Birmingham City just about showed their Championship rivals how it is done after Sebastian Larsson's 83rd minute goal secured a 1-0 home success over plucky Shrewsbury Town.
Freddy Eastwood proved to be the difference down at Dean Court as Southend United recorded a 3-1 victory over Bournemouth.
Mark Gower put the visitors ahead on 33 minutes before Bournemouth levelled two minutes into the second half through the evergreen Steve Fletcher.
But in-demand striker Eastwood restored Southend's advantage on 56 minutes via the penalty spot and he netted again to wrap up the win on 67 minutes.
Barnsley needed penalties to beat Blackpool at Bloomfield Road, with Andy Ritchie's men prevailing 4-2 in the shoot-out.
Robbie Williams' spot kick put The Tykes ahead, but Scott Vernon sent the match into extra time. Vernon also made penalties necessary with a dramatic late equaliser after Martin Devaney had put Barnsley back in front.
Luton Town also required a shoot-out win to progress as they won 5-3 on spot kicks at Bristol Rovers.
Adam Boyd had put The Hatters ahead after just three minutes, but a 61st minute goal from Richard Walker restored parity.
Darryl Duffy's 110th minute goal took Hull City into the next round after a 2-1 win at home to Tranmere Rovers. Shane Sherriff put the visitors ahead, but Ben Burgess levelled in the second half.
Cheltenham Town edged past fellow League One side Bristol City with a 2-1 triumph at Whaddon Road.
Stephen Guinan and Brian Wilson settled the tie in the hosts' favour, with David Cotterill replying for the away side.
Last season, Doncaster Rovers enjoyed a fine run in the competition and set about repeating those heroics with a 3-2 win at home to Rochdale.
The visitors nosed in front on the stroke of half time through Adam Rundle before two goals in the space of a minute from Mark McCammon and James Coppinger put Doncaster ahead.
McCammon scored again on 67 minutes, although Rochdale set up a frantic finish via John Doolan's 79th minute effort.
Carlisle United beat Bradford City 4-3 on penalties after a 1-1 draw at Brunton Park, in a game which was held up by floodlight failure.
Derek Holmes had put the hosts ahead on 31 minutes, but Eddie Johnson sent the game into extra time with an 81st minute leveller.
Goals from Nicky Maynard, Michael O'Connor and a Ryan Lowe penalty secured Crewe Alexandra a convincing 3-0 win at Grimsby Town.
Michael Boulding and Richie Barker netted either side of half time to reward Mansfield Town with an impressive 2-0 success at Huddersfield Town.
Click below for an individual match summary:
Birmingham City 1-0 Shrewsbury Town
Blackpool 2-2 (2-4 on pens) Barnsley
Bournemouth 1-3 Southend United
Bristol Rovers 1-1 (3-5 on pens) Luton Town
Burnley 0-1 Hartlepool United
Bury 2-0 Sunderland
Cardiff City 0-2 Barnet
Carlisle United 1-1 (4-3 on pens) Bradford City
Cheltenham Town 2-1 Bristol City
Crystal Palace 1-2 Notts County
Doncaster Rovers 3-2 Rochdale
Grimsby Town 0-3 Crewe Alexandra
Hereford United 3-1 Coventry City
Huddersfield Town 0-2 Mansfield Town
Hull City 2-1 (AET) Tranmere Rovers