Brighton & Hove Albion have seen a second bid for Virgil van Dijk knocked back by Eredivisie outfit FC Groningen.
An offer believed to be in the region of €2.4million (£2.1m) was rebuffed by Groningen once Brighton had made their interest known.
Gus Poyet has refused to give up on his efforts to land Van Dijk, with a fresh approach made for the Holland U21 international.
Groningen are, however, prepared to stand firm.
They have rejected the latest offer put to them and maintain that they have no intention of parting with the defender during the winter window.
"Received a renewed bid from Brighton for Virigil van Dijk. We have turned it down again," Groningen's general director Hans Nijland posted on his Twitter account.
He later told Voetbal International: "We have thanked them for their bid and let it be known that Virgil won't leave us during this winter.
"If it's up to us, they will not return with a new bid."